Table 2. Regression coefficients k, the coefficients of determination R² and the accuracy coefficient L² indicating the correspondence between the automatic and the manual counting for the B.subtilis live/dead ratios on fluorescent images.

	12 images			50 images
Threshold	k	R2	L2	k	R2	L2
15	1,02	0,92	1,00	1,08	0,72	0,97
20	1,09	0,93	0,97	1,11	0,74	0,95
30	1,07	0,91	0,98	1,17	0,75	0,90
45	1,10	0,84	0,96	1,14	0,74	0,93
60	1,14	0,90	0,93	1,10	0,74	0,96