Table 2. Predicted CTL epitopes from (HPV31 to HPV68) E6 protein.

Prediction score threshold was set at > 0.75000. Bold indicates amino acids that were also predicted as antigenic sites.

Residue No	Peptide Sequence	MHC Binding Affinity	Rescale Binding Affinity	C-terminal Cleavage Affinity	Transport Affinity	Prediction Score	MHC-I Binding
15	LSSALEIPY	0.4620	1.9614	0.6850	2.7980	2.2040	Yes
37	LTETEVLDF	0.5417	2.3000	0.8056	2.3240	2.5371	Yes
39	ETEVLDFAF	0.2203	0.9355	0.0724	2.1750	1.0551	Yes
47	FTDLTIVYR	0.1915	0.8130	0.8104	1.3460	1.0019	Yes
73	VSEFRWYRY	0.5615	2.3842	2.3842	2.9840	2.6666	Yes
39	RSEVYDFAF	0.2390	1.0147	0.1258	2.6260	1.1649	Yes
73	ISEYRHYNY	0.5954	2.5278	0.8555	2.9450	2.8033	Yes
2	FQDPAERPY	0.2014	0.8549	0.8643	2.7650	1.1228	Yes
73	ISEYRWYRY	0.5572	2.3657	0.8670	2.9450	2.6430	Yes
41	QTEVYEFAF	0.3068	1.3027	0.0842	2.3330	1.4320	Yes
45	YEFAFSDLY	0.1254	0.5323	0.9668	2.9280	0.8237	Yes
49	FSDLYVVYR	0.1983	0.8419	0.8143	1.4080	1.0345	Yes
72	YAKIRELRY	0.2013	0.8546	0.9407	2.8500	1.1382	Yes
81	YSDSVYATT	0.3351	1.4228	0.0616	0.9130	1.3863	Yes
95	NTKLYNLLI	0.1752	0.7439	0.7623	0.5580	0.8862	Yes
18	CTELNTSLQ	0.2302	0.9776	0.0277	-0.2640	0.9686	Yes
37	ATLERTEVY	0.3216	1.3654	0.9669	3.1660	1.6687	Yes
41	RTEVYQFAF	0.3261	1.3848	0.2831	2.4460	1.5495	Yes
72	YSRIRELRY	0.3333	1.4151	0.8846	2.8890	1.6922	Yes
95	NTELYNLLI	0.4589	1.9483	0.8040	0.4160	2.0897	Yes
39	RADVYNVAF	0.2034	0.8635	0.3223	2.6480	1.0442	Yes
89	EAITKKSLY	0.1378	0.5852	0.9040	2.8900	0.8653	Yes
47	FTDLRIVYR	0.1636	0.6948	0.5907	1.2400	0.8454	Yes
73	ISEYRHYQY	0.5593	2.3746	0.9645	2.9450	2.6666	Yes
76	YRHYQYSLY	0.1668	0.7084	0.9696	2.9740	1.0025	Yes
73	YSKVRKYRY	0.3031	1.2871	0.8705	2.8380	1.5596	Yes
99	LCDLLIRCY	0.1708	0.7250	0.8642	2.7240	0.9909	Yes
35	KTLQRSEVY	0.2565	1.0890	0.8691	3.1290	1.3758	Yes
39	RSEVYDFVF	0.2152	0.9138	0.2751	2.6260	1.0864	Yes
68	RLLSKISEY	0.1342	0.5698	0.9665	3.2540	0.8774	Yes
73	ISEYRHYNY	0.5954	2.5278	0.9588	2.9450	2.8188	Yes
76	YRHYNYSLY	0.1608	0.6829	0.9673	2.9740	0.9767	Yes
20	TLDTTLHDV	0.1793	0.7613	0.7970	0.2690	0.8943	Yes
41	RTEVYEFAF	0.2257	0.9584	0.1397	2.5640	1.1075	Yes
49	FSDLCVVYR	0.1559	0.6619	0.2967	1.4080	0.7768	Yes
72	YAKIRELRY	0.2013	0.8546	0.9002	2.8500	1.1322	Yes
91	ETITNTKLY	0.3233	1.3728	0.8601	2.8160	1.6426	Yes
95	NTKLYNLLI	0.1752	0.7439	0.8751	0.5580	0.9031	Yes