Fig 5. Absence of IFNAR signaling in Tregs results in defective generation of memory T cells in LCMV infection.

(A) Spleen cells of chronic LCMV infected (day 46) IFNAR^fl/fl and IFNAR^fl/fl x Foxp3^YFP-Cre mice were analyzed for frequencies and absolute numbers of GP276 Tet⁺ T cells. Frequencies and absolute numbers of CD62L⁺ (B), CD127⁺ (C), CXCR3⁺ (D), and KLRG1⁺ (E) cells were determined within gated CD8⁺CD44⁺GP276 Tet⁺ T cells (as in A). Splenocytes harvested on day 5 Cl-13 recall challenge after day 30 LCMV Armstrong infection (memory phase), and CD8⁺CD44⁺ T cells were analyzed for frequencies and absolute numbers of NP396 Tet⁺ T cells (F). Spleen cells from Cl-13 recall challenge were stimulated with GP33, GP276, and Golgi stop for 5 hours at 37 ^oC, frequencies and absolute numbers of CD8⁺CD44⁺ T cells producing Granzyme B, and IFN-γ were analyzed (G). * P < 0.05, ** P < 0.01, *** P < 0.001, and **** P < 0.0001 (unpaired two-tailed Student’s t-test). Data derived from one to two experiments involving three to eight mice per group (Mean±SEM).