Table 2. Sequence analysis of DENV passages.

Passage (P)		P 2			P 3			P 4		P 5
		DMSO	3ab	3bb	DMSO	3a	3b	DMSO	3a	DMSO
Transition	G→A		3	6	1	3	1		3
	A→G	2	3	6	1		4	1	2
	U→C		1	14	4	2	3		4	4
	C→U	2	4	4	1	3	6		3	2
Transversion	U→A	1	2				1		2
	G→U	1	2	2		1
	U→G	1	1	1
	C→A								2	1
	A→U		1
Insertion					1
Deletion					1
Number of different mutations		7	17	33	9	9	15	1	16	7
Number of total mutations		11	80	47	10	19	50	1	27	8
Number of nucleotides sequenced		38,829	32,508	30,702	36,120	33,411	31,605	40,635	40,635	35,217
Number of haplotypes		7	17	22	9	9	17	2	15	8
Number of clones with mutation/total		7/43	26/36	22/34	9/40	12/37	22/35	1/45	19/45	8/39
Mf mina, per 10,000 nucleotides		1.8	5.2	10.7	2.5	2.7	4.7	0.2	3.9	2.0
Mf maxa, per 10,000 nucleotides		2.8	24.6	15.3	2.8	5.7	15.8	0.2	6.6	2.3
Mfea (×10−3)		1.4	2.5	2.4	1.1	1.4	2.2	0.6	1.6	1.0

^aDefined in the text

^b3a 200 μM and 3b 500 μM for all passages