Table I.

Purification of CS from young developing tea leaves

Step	Fraction	Volume	Total Activity	Total Protein	Specific Activity	Purification	Yield
		mL	pkat	mg	pkat mg−1	fold	%
1	Crude extract	930	6330	581	10.9	1.0	100
2	Ammonium sulfate	33.8	3410	155	22.0	2.0	53.9
3	Hydroxyapatite	23.0	2630	28.9	91.0	8.0	41.5
4	Shodex IEC QA-824	7.5	1070	4.82	221	20.3	16.9
5	Adenosine-agarose	2.0	202	0.08	2530	232	3.2
6	Superdex 200	5.8	228	0.04	5700	523	3.6