Southern genome analysis of Synechococcus PIM8 transformed with pFP1-3, pFP1-3-crtB, pFP1-3-pys, and pFP1-3-crtZ. Hybridization was with a digoxygenin-labeled crtB probe (A), a pys probe (B), or a crtZ probe (C). DNA was digested with EcoRV (a), EcoRV and HindIII (b), or EcoRV and DraII (c). Sizes are indicated in bp.