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. 2018 Mar 22;8(5):764–773. doi: 10.1002/2211-5463.12408

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© 2018 The Authors. Published by FEBS Press and John Wiley & Sons Ltd.

This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.

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PCBP2 directly bound to CDK2 3′ UTR. (A) Biotinylated RNA pulldown assay. The biotinylated different regions of CDK2 mRNA were incubated with HGC‐27 cell lysates and the mRNA regions interacting with PCBP2 were analyzed using western blotting. Both PCBP2 and GAPDH protein levels are presented and GAPDH was used as a control. (B) RNP IP assay. mRNAs of CDK2 and negative control genes (HER2 and CDK1) were captured by anti‐PCBP2 antibody or control IgG and were analyzed using qRT‐PCR. The total input mRNAs of CDK2 and negative control genes (HER2 and CDK1) were also detected. **P < 0.01.