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. Author manuscript; available in PMC: 2018 Oct 3.
Published in final edited form as: Mol Nutr Food Res. 2018 Apr 3;62(8):e1700856. doi: 10.1002/mnfr.201700856

Figure 4.

Figure 4

PMI5011-mediated decrease in hepatic lipid accumulation is not associated with robust changes in gene or protein expression of factors regulating lipid metabolism. A) Expression of a panel of genes involved in lipid metabolism was assayed in mixed gastrocnemius muscle using qRTPCR. B,C) Total and phospho-protein levels were determined in the liver using a panel of proteins that regulate signaling events and transcriptional activity in fatty acid oxidation or insulin signaling in mice fed the HFD alone (Control) or supplemented with PMI5011 (PMI5011). D) Phosphorylation of AMPK relative to total AMPK (AMPKα1 and AMPKα2) and E) phosphorylation of AKT relative to total AKT was determined. F) H&E staining of the liver from mice fed a HFD alone (Control) or supplemented with PMI5011 (PMI5011). G) Triglyceride content was assayed in the liver and reported as mg/dl. Statistical significance is reported as the mean ± SD, compared to control; *p < 0.05, **p < 0.01