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. Author manuscript; available in PMC: 2018 Nov 1.
Published in final edited form as: J Immunol. 2017 Sep 20;199(9):3249–3260. doi: 10.4049/jimmunol.1700823

Figure 7. CRISPR KO of p205 leads to reduced Asc expression in B16 melanoma.

Figure 7

(A) Expression of p205 mRNA in IFN-stimulated wild type (WT) and p205 knockout (KO) B16 melanoma cell lines relative to Hprt and normalized to non-treated (NT). (B) p205 and β-actin protein expression in WT and p205 KO cell lines stimulated with Sendai virus (n.s.- non-specific band) (C) Mature mRNA and pre-mRNA profile of Asc expression (relative to Hprt and normalized to WT) and (D) Western blot analysis of Asc and β-actin in WT and p205 KO B16 melanoma cell lines.