Unsupervised principal component analysis (PCA) plot evaluating the development dnDSA, post-transplant d-sp T-cell alloreactivity in relation to kidney allograft function (serum creatinine) at 24 months. The unsupervised principal component analysis (PCA) segregated the patients into 2 different groups, those without d-sp T-cell alloreactivity (negative d-sp IFN-γ ELISPOT), absence of dnDSA and better preserved 24-month kidney allograft function from those transplant patients with high d-sp alloreactivity (positive d-sp IFN-γ ELISPOT), presence of de novo humoral immunity and worse 24-month allograft function. Percentages of variance were: 48.3% for principal component 1, 33% for principal component 2 and 10.9% for principal component 3 (PCA mapping= 92.2%).