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. Author manuscript; available in PMC: 2019 May 1.
Published in final edited form as: Int J Parasitol. 2018 Feb 10;48(6):423–431. doi: 10.1016/j.ijpara.2017.10.008

Fig. 2.

Fig. 2

Downregulation of the CDH3 and LOXL4 genes in INT and HCT-8 cells following Cryptosporidium parvum infection. (A–D) INT and HCT-8 cells were exposed to C. parvum infection for various periods of time, followed by real-time quantitative PCR analysis of CDH3 and LOXL4 RNA levels. Downregulation of both CDH3 and LOXL4 genes was confirmed in INT and HCT-8 cells following C. parvum infection (A–D, respectively). (E–F) Protein contents of CDH3 and LOXL4 in HCT-8 cells following C. parvum infection for 24, 36 and 48 h, as assessed by western blots. Representative gel images for CDH3 and LOXL4 protein blotting are shown and signal intensities on the blots were quantified by densitometry (E and F, respectively). Data represent three independent experiments. *P<0.01, ANOVA compared with non-infected cells.