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. Author manuscript; available in PMC: 2019 May 1.
Published in final edited form as: Int J Parasitol. 2018 Feb 10;48(6):423–431. doi: 10.1016/j.ijpara.2017.10.008

Fig. 3.

Fig. 3

Downregulation of the CDH3 and LOXL4 genes in epithelial cells following Cryptosporidium parvum infection is correlated with delivery of Cdg7_FLc_1000 into the infected host cells. (A) Inhibition of delivery of Cdg7_FLc_1000 into infected cells through pre-treatment of host cells with a small interfering RNA (siRNA)to Cdg7_FLc_1000 followed by exposure of cells to C. parvum infection. INT and HCT-8 cells were treated with an siRNA to Cdg7_FLc_1000 for 12 h and then exposed to C. parvum infection for an additional 24 h. Contents of Cdg7_FLc_1000 in the whole infected cells were quantified by real-time quantitative . A non-specific scrambled siRNA was used as the control (Ctrl). (B–C) Inhibition of Cdg7_FLc_1000 in host cells by the siRNA treatment attenuated the downregulation of CDH3 and LOXL4 following C. parvum infection. INT and HCT-8 cells were treated with an siRNA to Cdg7_FLc_1000 for 12 h and then exposed to C. parvum infection for an additional 24 h. Expression levels of CDH3 and LOXL4 in the infected cells were quantified by real-time quantitative PCR. Data represent three independent experiments. *P<0.01, ANOVA compared with non-infected cells treated with the control siRNA; # P<.01, ANOVA compared with infected cells treated with the control siRNA.