(A) Effects of scopolamine on phosphorylation of SFK Y416 and
expression of Fyn and Src. Rats were administered with scopolamine (5 mg/kg,
i.p.) and were sacrificed at a different time point (7.5, 15, or 30 min) after
scopolamine injection. (B) Effects of saline and scopolamine on
phosphorylation of SFK Y416 and expression of Fyn and Src. Rats were
administered with saline (Sal) or scopolamine (Sco, 5 mg/kg, i.p.) and were
sacrificed 1 or 3 h after saline or scopolamine injection. Representative
immunoblots are shown to the left of the quantified data. Note that scopolamine
induced a time-dependent increase in Y416 phosphorylation, while the antagonist
did not alter expression of total Fyn and Src proteins. Data are presented as
means ± SEM (n = 3–5 per group).
*P < 0.05 versus saline (0 min) (A) or saline at
the same time point (B).