A. IFN-γ release ELISpot for three patients with positive timepoint 1 results and subsequent timepoints.
B. CD137 T cell activation by flow cytometry of the three patients with positive timepoint 1 results above.
Abbreviations: PIP-TAZ, piperacillin-tazobactam; CEF, ceftriaxone; TEN, toxic epidermal necrolysis; SFU, spot forming units; DRESS, drug reaction with eosinophilia system symptoms.
Demonstration of IFN-y release responses in patients with an initial positive result (> 50 SFU/million cells) with a subsequent bleed at least 9 months’ post SCAR onset.
Timepoints post SCAR onset: S1 DRESS (Timepoint 1, 66 days; Timepoint 2, 548 days)
S2 DRESS (Timepoint 1, 312 days; Timepoint 2, 556 days)
S6 DRESS (Timepoint 1, 4 days; Timepoint 2, 286 days)