FIG 1 .

P. aeruginosa intracellular survival and generation of bleb niches in HeLa cells. (A) Human corneal epithelial cells (hTCEpi), (B and C) Bronchial epithelial cells (NuLi) (B) or HeLa cells (C) were infected with PAO1 or an exsA mutant expressing GFP constitutively (plasmid pSMC2). Amikacin (200 µg/ml) was added 3 h postinfection to eliminate extracellular bacteria. Cells were imaged at 7 h postinfection. Results of quantification of blebbing cells from approximately 200 cells per experiment are shown. ND, none detected. Bar, 50 µm.