FIG 3 .

Ofloxacin treatment limits the intracellular location of P. aeruginosa PAO1 and its effector-null mutant. (A) HeLa cells were infected with strain PAO1 transformed with T3SS-GFP reporter pJNE05. Extracellular bacteria were killed with 200 µg/ml amikacin at 3 h, and WGA-Alexa Fluor 647 was added for 15 min and removed prior to imaging. The maximum projection is shown along with a single slice with XZ and YZ projections. (B) HeLa cells were infected with strain PAO1 or mutant PAO1ΔexoSTY transformed with pJNE05. Extracellular bacteria were killed with 200 µg/ml amikacin at 3 h, and all bacteria were killed with amikacin in combination with 25 µg/ml ofloxacin, which is cell permeative. Imaging began at 4 h postinfection and continued to be performed at 1-h intervals until 16 h. Selected intervals are shown. Bar, 25 µm.