FIG 9 .

(A) Absorbance (OD₅₄₀) was used to measure the growth of P. aeruginosa strain PAO1 or mutant PA103ΔexoUT in TSB containing 100 mM MSG and 1% glycerol as well as EGTA (2 mM) for T3SS induction. Levels of absorbance at 540 nm and GFP fluorescence were measured, and the results seen at the 5-, 10-, and 15-h time points are shown as bar graphs. (B) The indicated strains expressing the T3SS reporter were grown in TSB or in inducing media up to 8 h, fixed with paraformaldehyde, stained with propidium iodide (PI), and imaged. PI is indicated in red and GFP in green. (C) The levels of GFP intensity of individual bacteria were quantified from the images represented in panel B. The bars show the medians and interquartile ranges of the data from the indicated populations.