TABLE 1 .
Strains and plasmids used in this study
| Strain or plasmid | Description | Source or reference |
|---|---|---|
| Strains | ||
| PAO1 | Wild type | Arne Riestch (Case Western Reserve University) |
| PAO1ΔexsA | exsA mutant | Arne Riestch (Case Western Reserve University) |
| PAO1ΔexoSTY | exoS, exoT, and exoY mutant | Arne Riestch (Case Western Reserve University) |
| PAO1ΔfleQ | fleQ mutant | This study |
| PAO1ΔexoSTYΔfleQ | exoS, exoT, exoY, and fleQ mutant | This study |
| PAK | Wild type | Joanne Engel (University of California, San Francisco) |
| PAKΔexoSTY | exoS, exoT, and exoY mutant | Joanne Engel (University of California, San Francisco) |
| PA103ΔexoUT | exoU and exoT mutant | Dara Frank (Medical College of Wisconsin) |
| PA103ΔexoUT+fleQ | exoU and exoT mutant with restored fleQ (T719G) | This study |
| PA14ΔexoUTY | exoU, exoT, and exoY mutant | Gerald Pier (Harvard Medical School) |
| mPAO1 | Transposon mutant library parent | PAO1 transposon mutant library (44) |
| mPAO1 tn-fliC | PW8407 fliC-B03::ISphoA/hah | PAO1 transposon mutant library (44) |
| mPAO1 tn-flhA | PW3636, flhA-E11::ISlacZ/hah | PAO1 transposon mutant library (44) |
| mPAO1 tn-fleQ a | PW2981, fleQ-G06::ISphoA/hah | PAO1 transposon mutant library (44) |
| mPAO1 tn fleQ b | PW2980, fleQ-C04::ISphoA/hah | PAO1 transposon mutant library (44) |
| Plasmids | ||
| pUCP18 | P. aeruginosa expression vector | Joseph Barbieri (Medical College of Wisconsin) |
| pExoS (pUCP18 ExoS-HA) | ExoS expression vector | Joseph Barbieri (Medical College of Wisconsin) |
| pJNE05 | T3SS-GFP reporter | Timothy Yahr (University of Iowa) (35) |
| p67T1 | dTomato expression vector | John Singer (University of Maine) (36) |
| pEXG2 | Integrating suicide plasmid | Arne Riestch (Case Western Reserve University) |