TABLE 1.
Level of diversityb | Index of diversity by community |
Microbial cooccurrenced |
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Bacteria |
Fungi |
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Richnessc | Shannon index | Evenness index | Richness | Shannon index | Evenness index | Nb nodes | Total no. of links | No. of bacterial links | No. of fungal links | B/F | P/N | |
Native | 1,004 ± 98.8 A | 5.48 ± 0.13 A | 0.79 ± 0.01 A | 449 ± 54.8 A | 3.87 ± 0.21 AB | 0.63 ± 0.07 A | 446 | 16,822 | 8,099 | 1,668 | 4.86 | 1.09 |
D1 | 659 ± 19.4 B | 4.20 ± 0.09 B | 0.65 ± 0.02 B | 462 ± 51.8 A | 3.95 ± 0.31 A | 0.64 ± 0.04 A | 331 | 9,524 | 3,222 | 1,718 | 1.88 | 1.12 |
D2 | 435 ± 110.7 C | 3.62 ± 0.54 B | 0.59 ± 0.06 B | 356 ± 51.5 A | 3.32 ± 0.29 AB | 0.57 ± 0.04 A | 186 | 3,718 | 1,499 | 532 | 2.89 | 1.07 |
D3 | 313 ± 56.6 C | 2.97 ± 0.40 C | 0.51 ± 0.05 C | 194 ± 91.0 B | 2.51 ± 1.42 B | 0.47 ± 0.22 A | 107 | 1,367 | 593 | 219 | 2.71 | 1.09 |
Values were calculated from the sequencing data for the native community and for each level of diversity at T0 (i.e., the end of the 6 weeks of preincubation). For each level of diversity, values with different letters differ significantly (P < 0.05). Values are expressed as means ± standard deviation (n = 3 biological replicates).
D1, high; D2, medium; D3, low.
Richness refers to the number of OTUs defined at the genus level.
Nb nodes, number of taxa connected in the network; bacterial links and fungal links, links involving, respectively, bacteria only and fungi only; B/F, ratio between the numbers of bacterial and fungal links; P/N, ratio between the numbers of positive and negative links in the cooccurrence network.