FIG 1.

De novo polymerization activity of the recombinant Gsy. (A) Morphology of E. coli strains carrying pET28a(+)-gsy (left), compared to those carrying the empty vector (right). (B) SDS-PAGE analyses. (Left) Total protein extracts of E. coli cells were analyzed by SDS-PAGE, followed by staining with Coomassie brilliant blue (CBB). Note that a differentially expressed protein band was apparent in samples induced with IPTG. (Right) An equivalent gel was subjected to an in situ polymerization assay. At the site similar to the differential band in the CBB-stained gel, a polysaccharide-active band of ∼170 kDa was obvious.