FIG 6.

Phylogenetic analysis of the four subgroups of GH70 enzymes and GH13 proteins. Alignment was performed with the GH70 and GH13 enzymes listed in Table S1 in the supplemental material. Overall, the GH70 enzymes were obtained by a BLAST analysis using the Gsy protein (GenBank accession number ANJ45894.1) as the query sequence. Notably, all of the characterized GH70 enzymes (http://www.cazy.org/GH70_characterized.html) were included in the alignment. Members of GH13 in the alignment were chosen according to previous studies (7, 19). The evolutionary tree is based on alignment of the full sequences of these enzymes. The biochemically characterized GSs and GTFB-like and GTFC-like 4,6-α-GTs of GH70, as well as members of GH13, are included. The evolutionary history was inferred using the maximum likelihood method, based on the JTT matrix-based model. The sequences are labeled with their GenBank accession numbers. The bar represents a genetic distance of 0.3 substitution per position. The BrS and GS subgroups of GH70 are indicated in green and red fonts, respectively. Gsy is shown in red font shaded with yellow, and its homologs that have not been biochemically characterized are shown in gray.