FIG 5.

Effect of Ca²⁺ on the NO signaling pathway under HS treatment conditions. (A) The WT strains were cultured on PDA plates for 5 days, treated with 2 mM CaCl₂ or 500 μM EGTA for 30 min, and then exposed to 42°C for 20 min. The changes in NO levels were detected by fluorescence microscopy after staining with DAF-FM DA. (B) Changes in the NO fluorescence ratios in the strains subjected to different treatments. (C) The WT and CaMi strains were cultured on PDA plates for 5 days, treated with or without 2 mM CaCl₂ for 30 min, and then exposed to 42°C for 20 min. The changes in NO levels were detected by fluorescence microscopy after staining with DAF-FM DA. (D) Changes in the NO fluorescence ratios in WT and CaMi strains subjected to different treatments. (E) Effect of CaCl₂ and EGTA on the expression of the NR gene in G. lucidum under HS treatment. (F) Changes in the expression of the NR gene in the WT and CaMi strains under HS treatment with or without CaCl₂. The values are the means ± SD of the results from three independent experiments. Different letters indicate significant differences between the lines (P < 0.05, according to Duncan's multiple-range test).