FIG 6.

Effects of Ca²⁺ on GA biosynthesis in the WT and NRi strains. The WT strains were cultured in PDA liquid cultures with shaking for 5 days with the addition of exogenous 2 mM CaCl₂ and 500 μM SNP in the presence of 500 μM EGTA and then exposed to 42°C for 12 h. (A to C) Measurements of the total GA (A), squalene (B), and lanosterol (C) contents in heat-stressed strains are shown. The WT and NRi strains were cultured in PDA liquid cultures with shaking for 5 days, and exogenous 2 mM CaCl₂ and 500 μM SNP were added in the presence of 50 μM W7. (D to F) Measurements of the total GA (D), squalene (E), and lanosterol (F) contents in the heat-stressed strains are shown. The values are the means ± SD of the results from three independent experiments. Different letters indicate significant differences between the lines (P < 0.05, according to Duncan's multiple-range test).