FIG 7.

Effects of NO on GA biosynthesis in the WT and CaMi strains. The WT and CaMi strains were cultured in PDA liquid cultures with shaking for 5 days, and exogenous 2 mM CaCl₂ and 500 μM SNP were added in the presence of 500 μM cPTIO, respectively. The strains were then exposed to 42°C for 12 h. (A to C) Measurements of the total GA (A), squalene (B), and lanosterol (C) contents in the heat-stressed strains are shown. The WT and CaMi strains were cultured in PDA liquid cultures with shaking for 5 days, and exogenous 2 mM CaCl₂ and 500 μM SNP were added in the presence of 500 μM Na₂WO₄. The strains were then exposed to 42°C for 12 h. (D to F) Measurements of the total GA (D), squalene (E), and lanosterol (F) contents in the heat-stressed strains are shown. The values are the means ± SD of three independent experiments. Different letters indicate significant differences between the lines (P < 0.05, according to Duncan's multiple-range test).