FIG 2.

Boxshade representation of multiple-sequence alignment of 12α-hydroxysteroid dehydrogenases from Eggerthella lenta strains and Clostridium species. Depicted in the alignment are 12α-HSDH from Eggerthella lenta DSM 2243 (Elen_2515) (33), Eggerthella CAG:298 (accession no. CDD59475) (34), Clostridium sp. strain ATCC 29733 (ClostATCC29733), Clostridium scindens ATCC 35704 (CSCI), Clostridium hylemonae DSM 15053 (CHYL), and Clostridium hiranonis DSM 13275 (CHIR). Asterisks mark amino acids predicted to bind pyridine nucleotide cofactor, and diamonds indicate conserved active-site residues in the SDR family.