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. 2018 May 1;84(10):e00235-18. doi: 10.1128/AEM.00235-18

FIG 5.

FIG 5

Optimal pHs for purified recombinant 12α-hydroxysteroid dehydrogenases. Substrates were 12-oxoLCA with NADPH as cofactor (reductive direction, red) or DCA with NADP+ as cofactor (oxidative direction, blue). See Materials and Methods for buffer compositions. Experiments were repeated three or more times; values represent means ± standard deviation (SD).