TABLE 1.
Oxygen consumption by intact cells or dialyzed crude cell extracts of JS678
| Assay substrate(s)a | Oxygen consumption (nmol O2/min/mg protein [mol O2/mol of substrate])b |
|||
|---|---|---|---|---|
| Whole cells |
Dialyzed cell extract |
|||
| Succinate grown | Resveratrol grown | Succinate grown | Resveratrol grown | |
| Resveratrol | 13 ± 1.5 | 437 ± 4.5 (6.71 ± 0.22) | NDc | 620 ± 15 |
| 4-Hydroxybenzaldehyde | ND | 186 ± 1.5 (3.49 ± 0.09) | ND | ND |
| 3,5-Dihydroxybenzaldehyde | ND | 328 ± 0.2 (3.31 ± 0.27) | ND | ND |
| 4-Hydroxybenzoate | ND | 239 ± 3 (2.57 ± 0.34) | ND | ND |
| 3,5-Dihydroxybenzoate | ND | 6 ± 0.5 (2.51 ± 0.18) | 2 ± 0.1 | ND |
| 3,4-Dihydroxybenzoate | ND | 293 ± 12.1 (1.51 ± 0.06) | ND | 440 ± 15 |
| Gentisic acid | ND | ND | ND | ND |
| Hydroxyhydroquinone | ND | ND | ND | ND |
| Succinate | 161 ± 1 | 320 ± 1.5 | 140 ± 0.7 | ND |
| trans-Stilbene | ND | 3 ± 0.2 | ND | ND |
| Pterostilbene | ND | ND | ND | ND |
| Arachidin-3 | ND | ND | ND | ND |
| 4-Hydroxybenzaldehyde, NAD+ | NMd | NM | ND | ND |
| 3,5-Dihydroxybenzaldehyde, NAD+ | NM | NM | 1.8 ± 0.1 | 110 ± 10 |
| 4-Hydroxybenzoate, FAD, NADPH | NM | NM | ND | 110 ± 6 |
| 4-Hydroxybenzoate, NADPH | NM | NM | ND | ND |
| 3,5-Dihydroxybenzoate, NADPH | NM | NM | ND | ND |
| 3,5-Dihydroxybenzoate, NADH | NM | NM | ND | 127 ± 11 |
a
Substrates were added at a concentration of 100 μM, and two replicates were performed per experiment.
b
Data represent the means of duplicate analyses; the errors were calculated by taking the standard deviations between the data points.
c
ND, not detected.
d
NM, not measured.