TABLE 1.
Primers used for promoter engineering
| Name | Sequence (5′→3′) | Construction |
|---|---|---|
| PPM1F | CGACCCGCCGATTGGTACATACATACATACATACATACAAGGATTCCGACAAAGG | P1pimM |
| PPM1R | CCTTTGTCGGAATCCTTGTATGTATGTATGTATGTATGTACCAATCGGCGGGTCG | P1pimM, P6pimM |
| PPM2F | CGGGAACGACGGTACATACATACAAGGAGCCGCCAGCG | P2pimM |
| PPM2R | CGCTGGCGGCTCCTTGTATGTATGTACCGTCGTTCCCG | P2pimM |
| PPM3F | GGTCCCGGGAACGTGGCAAGAAAGCGGCAGGTGTTCGGCAAGGATTCCACCAGGACCC | P3pimM |
| PPM34R | CGTTCCCGGGACCTTTGTC | P3pimM, P4pimM |
| PPM4F | GTCCCGGGAACGACGGCAGGCGAACGGC | P4pimM |
| PPM5F | GCGCCTTCCCGGCTGGCTGGCAAGAAAGTGGCAAGAAAGCGGCAGGTGTTCG | P5pimM |
| PPM5R | GCCAGCCGGGAAGGCGCTTTC | P5pimM |
| PPM6F | CAAAGGTCCCGGGAACGATGGCAAGAAAGCGGCAGGTGTTCGGCAAGGATTCCCCAGGACCC | P6pimM |
| PPM7F | GCGACCCGCCGATTACATACAAAGCGGCAGGTGTTCGGCAAGG | P7pimM |
| PimM-F | CGGTCCCCGTGCCTCTCGC | All except P7pimM |
| PimM-R | CGGCGAGGAGGCTTCAGC | All |