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. 1999 Jul;120(3):665–674. doi: 10.1104/pp.120.3.665

Figure 3.

Figure 3

Expression of zmGRP4 in maize tissues. A, Northern-hybridization analysis. Poly(A+) RNA (1.5 μg) was isolated from 2- to 3-cm-long roots, 3-d-old etiolated shoots, and 3-d-old shoots, and leaves of 2-week-old plants. A maize ubiquitin probe served as a control to estimate the relative loading of RNA in each lane. B, RT-PCR analysis. S, Shoot; ES, etiolated shoot; root proper, 0- to 0.5-, 0.5- to 1.0-, and 1.0- to 1.5-cm regions distal from the excision site; RT, root tip; NT, negative control without reverse transcription. A maize α-tublin gene (TUA) served as a positive control.