Figure 6.

Immunoblot analysis of zmGRP4. Each lane was loaded with 10 μg of protein extracted from the root tip or root proper. A, Total protein fraction (lanes T), Tris-buffer-soluble fraction (lanes S), and Tris-buffer-insoluble but SDS-buffer-soluble fraction (lanes I) were separated on a 15% SDS-polyacrylamide gel. B, The Tris-buffer-soluble fraction (S) and Tris-buffer-insoluble but SDS-buffer-soluble fraction (I) were extracted from intact root tips (+) or root tips from which mucilage had been removed (−).