Table II. Sulfenylated human microsomal proteins. Proteins with respective gene names identified in microsomes prepared from human livers and kidneys, at 5% peptide FDR. Modifications are presented as cysteine (C) and sequence location, with the percentage sulfenylation (±SD, n = 5 different tissue samples) indicated in parentheses.
| Description | Gene Name | Modifications Found in Liver | Modifications Found in Kidney | ||||
|---|---|---|---|---|---|---|---|
| Arylacetamide deacetylase | AADAC | C340 (28 ± 5%) | – | ||||
| Alcohol dehydrogenase 1A | ADH1A | C1331 (32 ± 2%) | C328 (31 ± 6%) | C1714 | C212 | – | |
| C1122 (35 ± 4%) | C98 (29 ± 8%) | C1754 | C47 | ||||
| C2413 (37 ± 3%) | C196 | ||||||
| Alcohol dehydrogenase 1B | ADH1B | C47 (27 ± 3%) | C1122 | C1754 | – | ||
| C985 (34 ± 2%) | C2413 | C1966 | |||||
| C1331 | C1714 | C2126 | |||||
| Alcohol dehydrogenase 1C | ADH1C | C1122 | C985 | C1967 | C2413 | – | |
| Alcohol dehydrogenase 4 | ADH4 | C246 (28 ± 2%) | C23 (37 ± 4%) | C287 | – | ||
| C274 (39 ± 5%) | C99 (32 ± 4%) | C2016 | |||||
| C47 (35 ± 3%) | C105 | C2176 | |||||
| Alcohol dehydrogenase 6 | ADH6 | C1967 | – | ||||
| Aldehyde dehydrogenase family 16 member A1 | ALDH16A1 | C350 | C496 | ||||
| Aldehyde dehydrogenase family 1 member A3 | ALDH1A3 | – | C196 | C197 | |||
| Aldehyde dehydrogenase, mitochondrial | ALDH2 | C386 (33 ± 3%) | C66 | C472 | C66 | C472 | |
| Aldehyde oxidase | AOX1 | – | C386 (23 ± 13%) | C179 | |||
| Liver carboxylesterase 1 | CES1 | C285 (40 ± 3%) | C390 (37 ± 1%) | C116 (37 ± 3%) | C390 | ||
| C274 (37 ± 5%) | |||||||
| Steroid 17 α-hydroxylase/17,20 lyase | CYP17A1 | – | C442 | ||||
| P450 1A2 | CYP1A2 | C504 (42 ± 5%) | C159 (44 ± 5%) | – | |||
| P450 26A1 | CYP26A1 | – | C170 | ||||
| Sterol 26-hydroxylase, mitochondrial | CYP27A1 | C228 (36 ± 6%) | C427 (43 ± 1%) | C228 | |||
| P450 2A6 | CYP2A6 | C82 (34 ± 4%) | C439 (36 ± 3%) | – | |||
| P450 2B6 | CYP2B6 | C436 (32 ± 3%) | C180 (27 ± 7%) | C152 (46 ± 2%) | C79 | – | |
| P450 2C18 | CYP2C18 | C3388 (42 ± 2%) | C216 (38 ± 4%) | C486 | C26610 | – | |
| C1519 (38 ± 2%) | |||||||
| P450 2C19 | CYP2C19 | C3388 | C372 (40 ± 4%) | C216 | C1519 | – | |
| C435 | C26610 | C179 | |||||
| P450 2C8 | CYP2C8 | C435 (37 ± 3%) | C26610 (39 ± 3%) | C216 | C172 | C26610 | |
| C1519 (41 ± 3%) | C486 (37 ± 4%) | C225 | C175 | ||||
| C51 (34 ± 2%) | C3388 | C179 | C164 | ||||
| P450 2C9 | CYP2C9 | C372 (38 ± 1%) | C216 (38 ± 3%) | C164 | C3388 | C1519 | C216 |
| C486 (38 ± 2%) | C172 | C179 | C1519 | ||||
| C266 (41 ± 5%) | |||||||
| P450 2D6 | CYP2D6 | C443 (45 ± 7%) | C191 (39 ± 4%) | C57 (27 ± 7%) | – | ||
| P450 2E1 | CYP2E1 | C261 (39 ± 6%) | C488 (34 ± 2%) | C480 (39 ± 4%) | – | ||
| C437 (37 ± 3%) | C268 (42 ± 2%) | ||||||
| P450 3A4 | CYP3A4 | C9811 (33 ± 1%) | C46812 (40 ± 2%) | C58 | – | ||
| P450 3A5/3A7 | CYP3A5/A7 | C9811 | C46712 | – | |||
| P450 4A11 | CYP4A11 | C25613 (40 ± 2%) | C200 (33 ± 4%) | C51314 | C375 | C25613 (41 ± 5%) | C86 |
| C53 (36 ± 5%) | C86 (36 ± 4%) | C200 (38 ± 6%) | C51314 | ||||
| P450 4A22 | CYP4A22 | C25613 | C51314 | C25613 | C51314 | ||
| P450 4F11 | CYP4F11 | C354 (36 ± 2%) | C46817 (43 ± 6%) | C38418* | C260 | C38418* | |
| C5016 | C102 (84 ± 5%) | C276 | |||||
| P450 4F12 | CYP4F12 | C35415 | C46817 | C384 | C5016 | – | |
| P450 4F2 | CYP4F2 | C102 (37 ± 2%) | C5019 (36 ± 6%) | C35415 | C468 | C35415 (43 ± 5%) | C102 |
| C384 (43 ± 13%) | |||||||
| P450 4F22 | CYP4F22 | C5819 | – | ||||
| P450 4F3 | CYP4F3 | C35415 | C402 | C38418* | C46817 | C35415 | C38418* |
| C50 (38 ± 2%) | |||||||
| P450 4F8 | CYP4F8 | – | C35415 | C38418* | |||
| P450 4V2 | CYP4V2 | C282 (37 ± 5%) | C383 (37 ± 5%) | C483 (40 ± 4%) | C406 | – | |
| P45051A1 | CYP51A1 | C337 | C345 | C366 (31 ± 6%) | C449 | – | |
| Epoxide hydrolase 1 | EPHX1 | C80 (39 ± 1%) | C304 (38 ± 7%) | C232 (39 ± 2%) | C80 (43 ± 4%) | C304 (46 ± 14%) | |
| C182 (38 ± 1%) | C182 (41 ± 3%) | C232 | |||||
| Dimethylaniline monooxygenase | FMO1 | – | C63 (43 ± 4%) | C111 (33 ± 3%) | |||
| [N-oxide-forming] 1 | C423 | ||||||
| Dimethylaniline monooxygenase | FMO3 | C170 (38 ± 1%) | C466 (43 ± 4%) | C68 (37 ± 3%) | C21 | C294 | |
| [N-oxide-forming] 3 | C397 (36 ± 1%) | C197 (41 ± 2%) | C294 (36 ± 9%) | C30 | |||
| C146 (36 ± 4%) | |||||||
| Dimethylaniline monooxygenase | FMO5 | C112 (35 ± 4%) | C468 (29 ± 10%) | C147 | – | ||
| [N-oxide-forming] 5 | C248 (28 ± 0%) | C31 | C22 | ||||
| Amine oxidase [flavin-containing] A | MAOA | C37421* (39 ± 2%) | C165 (32 ± 1%) | C306 (31 ± 2%) | C266 | C20120 (40 ± 4%) | C165 |
| C20120 (35 ± 1%) | C37421*(42 ± 4%) | C266 | |||||
| Amine oxidase [flavin-containing] B | MAOB | C156 (36 ± 1%) | C297 (37 ± 1%) | C36521* | C19220 | C297 (37 ± 1%) | C19220 |
| C156 (41 ± 3%) | C36521* | ||||||
| Serum paraoxonase/arylesterase 1 | PON1 | C42 (29 ± 2%) | C284 | – | |||
| Serum paraoxonase/lactonase 3 | PON3 | C42 (38 ± 7%) | – | ||||
| UDP-glucuronosyltransferase 1–4 | UGT1A4 | C187 (37 ± 4%) | – | ||||
| UDP-glucuronyltransferase 1–6 | UGT1A6 | C176 | C176 | ||||
| UDP-glucuronyltransferase 1–7 | UGT1A7 | – | C174 | ||||
| UDP-glucuronyltransferase 1–9 | UGT1A9 | – | C153 | ||||
| UDP-glucuronyltransferase 2A3 | UGT2A3 | – | C31 | ||||
| UDP-glucuronyltransferase 2B10 | UGT2B10 | C28122 (40 ± 4%) | – | ||||
| UDP-glucuronyltransferase 2B15 | UGT2B15 | C28322 | – | ||||
| UDP-glucuronyltransferase 2B17 | UGT2B17 | C28322 | – | ||||
| UDP-glucuronyltransferase 2B4 | UGT2B4 | C127 (28 ± 6%) | C514 | – | |||
| UDP-glucuronyltransferase 2B7 | UGT2B7 | C282 (35 ± 5%) | – | ||||
Shared peptide sequences: 1RFTCR, 2NPESNYCLK, 3ELGATECINPQDYK, 4VCLIGCGFSTGYGSAVNVAK, 5VIPLFTPQCGK, 6VTPGSTCAVFGLGGVGLSAVMGCK, 7VTPGSTCAVFGLGGVGLSVVMGCK, 8SPCMQDR, 9CLVEELRK, 10DFIDCFLIK, 11ECYSVFTNR, 12VLQNFSFKPCK, 13ACQLAHQHTDQVIQLR, 14RLPNPCEDKDQL, 15HPEYQEQCR, 16LQCFPQPPK, 17NCIGQAFAMAEMK, 18EIEWDDLAQLPFLTMCI(/L)K, 19LRCFPQPPR, 20QCGGTTR, 21I(/L)CELYAK, 22PFLPNVDFVGGLHCK.
* Single amino acid variation (leucine/isoleucine) in peptide sequences that cannot be determined from MS/MS fragmentation.