Fig. 5.

PYK2 is a SUMOylated tyrosine kinase. A, Bioactive peptide induced signaling pathway is enriched for SUMOylation substrates (p < 1.637 E10–4). Proteins that were recovered in our global SUMOylation assay are marked as SUMO modified. B, Validation of PYK2 SUMOylation and E3 ligase specificity in HeLa cells as indicated in Fig. 4A. SUMOylation of nonreceptor tyrosine kinase PYK2 was performed according to the conditions indicated in the global SUMOylation screen. C, Schematic representation of candidate attachment site lysines in PYK2. D, SUMOylation assay using PYK2 containing the four candidate lysine to arginine mutations (Fig 5C) demonstrated impaired SUMOylation in the 4KR mutant.