Fig. 6.

TIP for CD95/FAS-complex purification from primary human CD4⁺ T cells. A, Scheme describing the treatment of primary human CD4⁺ T cells before CD95/FAS-TIP isolation using 3 × 10⁷ untreated (-) or CD95L stimulated (+) cells. B, Western blot analysis for the indicated proteins isolated from nonstimulated (-) and CD95L stimulated (+) primary human CD4⁺ T cells by CD95/FAS TIP (LC: biotinylated mouse IgG light chain; arrowheads: specific signals). C, Venn diagram of the proteins identified and quantified by MaxQuant analysis comparing the isotype control-TIP and anti-CD95/FAS-TIP in resting and CD95L-treated samples. The list shows the proteins selectively enriched in the anti-CD95/FAS samples (ordered by LFQ values). The proteins in bold represent published complex components (for the MS results see supplemental Table S7). D, Sequence coverage of the peptides identified for PPM1G (left) and IPO7 (right). Bold letters indicate the peptides identified by LC-MS/MS.