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. 2018 Mar 9;14(4):369–380. doi: 10.7150/ijbs.24117

Figure 5.

Figure 5

Validation of differentially expressed genes with QPCR and western blot. (A, B and C), Expression level of selected titin direct binding partners with DNA microarray and QPCR; (D, E and F), Expression level of selected titin indirect binding partners with DNA microarray and QPCR; (G, H and I), Expression level of selected genes that are not titin binding partners with DNA microarray and QPCR; (J), Validation of selected proteins from titin direct and indirect partners and non-titin binding partners with western blot. D, day; Wt, wild type; Hm, homozygous Rbm20 knockout; GAPDH, protein loading control. Mean±SEM (n=3); *P<0.05; **P<0.01; ***P<0.001; #P<0.05; ##P<0.01; ###P<0.001.