Fig. 1.

Effects of propolis ethanol extract (PEE) on the number of eosinophils, macrophages, and myeloid-derived suppressor cells (MDSCs) in the mesenteric adipose tissue of ob/ob mice. PEE (100 mg/kg, twice weekly) or vehicle was intraperitoneally injected into ob/ob mice for one month. Eosinophils, CD11b⁺, Gr-1⁻ macrophages, and CD11b⁺, Gr-1⁺ MDSCs were subsequently detected by FACS. a Eosinophils in the living cells. b Macrophages and MDSCs in F4/80⁺ myeloid cells. (Left scatter plots) Representative FACS images of four replicates. (Right graphs) Proportion of eosinophils in the living cells (a) and macrophages and MDSCs in F4/80⁺ myeloid cells (b). Data represents means ± SD of four samples. *P < 0.05 vs. vehicle-treated mice