Fig. 6.

Effects of resinous compounds on the expression of MDSC markers in cultured macrophages. a-c Thioglycolate-elicited macrophages or (d, e) J774.1 cells were treated with vehicle, PEE (100 μg/mL), or twelve resinous compounds involved in PEE for 24 h and subsequently subjected to qRT-PCR analysis. a Comparisons of the effects of the compounds on Ly6g expression. Doses of each compound are described in the Results. b-e Effects of kaempferol (b, 0.03, 0.1, or 1 μg/mL; c-e, 0.1 μg/mL) on (b, d) Ly6g and (c, e) Il10 expression in (b, c) peritoneal macrophages and (d, e) J774.1 cells. Values were normalized to Hprt1 mRNA levels, and represented as means ± SD of (a) 4, (b) 3–4, (c) 3–5, or (d, e) 7 samples