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. 2018 May 1;11:34. doi: 10.1186/s13048-018-0410-3

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© The Author(s). 2018

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 2 — SMAD7 and ACVR2A are the direct targets of the miR-424/503 cluster members. Relative luciferase activity in bovine granulosa cells co-transfected with pmiRGLO vector harbouring the wild-type (WT) or mutant (MT) 3´-UTR sequence of SMAD7 or ACVR2A and the corresponding miRNA mimics or negative controls (NC) for miR-424 and miR-503. The data are presented as the mean ± SEM (*p < 0.05, **p < 0.01)