Fig. 6.

Sequence-specific knockdown of SMAD7 increased granulosa cell proliferation. The mRNA (a) and protein expression level (e) of SMAD7, mRNA level of PCNA (c), mRNA (d) and protein expression level (g) of STAR in granulosa cells transfected with SMAD7 siRNA or siRNA negative control (NC). The proliferation activity in granulosa cells transfected with SMAD7 siRNA (b). The protein expression level of SMAD2/3 and PSMAD2/3 in granulosa cells transfected with SMAD7 siRNA or siRNA negative control (NC) (f). Flow cytometric analysis showing the cell cycle distribution (G1/G0, S and G2/M phases) of propidium iodide (PI)-labeled bovine granulosa cells transfected with the SMAD7 siRNA (i) or siRNA negative control (NC) (h). Bar graphs showing the percentages of cells in G1/G0, S and G2/M phases of the cell cycle in cells transfected SMAD7 siRNA or siRNA negative control (NC) (j). The bar graphs indicate the mean ± SEM (**p < 0.01). Numbers above western blots represent relative protein density