Table 2.
Cell lines tested for replication of ancML
| Cell Line | Organism | Lineage | GFP# | NEOa | L1.3b | ancMLc |
|---|---|---|---|---|---|---|
| DF-1 | Gallus gallus | Aves | *** | +++ | ++ | − |
| CRFK | Felis catus | Carnivora | ** | +++ | + | − |
| CV-1 | Cercopithecus aethiops | Primates | * | ++ | + | − |
| Vero | Cercopithecus aethiops | Primates | *** | +++ | − | + |
| HT1080 | Homo sapiens | Primates | ** | ++ | + | − |
| HOS | Homo sapiens | Primates | * | ++ | − | − |
| Huh7.5 | Homo sapiens | Primates | * | ++ | − | − |
| HeLa | Homo sapiens | Primates | *** | +++ | ++ | − |
| pgsA745 | Cricetulus griseus | Rodentia | ** | ++ | + | ++ |
| CHO-K1 | Cricetulus griseus | Rodentia | ** | ++ | + | ++ |
| MusDunni | Mus dunni | Rodentia | *** | +++ | + | − |
| SC-1 | Mus musculus | Rodentia | ** | ++ | − | − |
| NIH3T3 | Mus musculus | Rodentia | ** | ++ | ++ | − |
All cell lines were transfected using lipofectamine 2000
#A plasmid expressing GFP was utilized as a transfection control. Percentage of GFP positive cells: (−) 0%, (*) < 10%, (**) 10–50%, (***) > 50%
aA pCR3.1 plasmid expressing a neo gene from a SV40 promoter was used as a control for G418 resistant colony formation
bThe replication dependent LINE1 (L1.3) plasmid [41] was used as a control for retrotransposition and G418 resistance from the interrupted NEO reporter cassette
cancML correspond to a pUC57 plasmid containing the cassette depicted in Fig. 3a
Number of G418 resistant colonies: (−) None, (+) ≤ 10, (++) > 10, (+++) > 50