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Quantitation of extracellular alkaline phosphatase activity in wild-type and mutant cultures. All strains were grown in TAP medium to early logarithmic phase, washed twice with TA medium, and then transferred to either TAP (A) or TA (B) liquid medium for 16, 25, 40, and 48 h before measuring the alkaline phosphatase activity. ◊, Wild type; ▪, psr2; ▵, psr1-1; ○, psr1-2; asterisks, psr1-2psr2. PNPP, p-Nitrophenylphosphate; chl, chlorophyll.
