Fig. 7.

PCNP mediates the PI3K/AKT/mTOR pathway in human neuroblastoma cells. a Western blotting analysis of the expressions of PI3K, p-PI3K (Tyr458/Tyr199), AKT, p-AKT (Ser473), mTOR, and p-mTOR (Ser2448) in SH-SY5Y and SK-N-SH cells. GAPDH was used as the loading control. b, c The intensities of the bands were quantified by densitometry analyses and normalized by the amount of PI3K, AKT, or mTOR. Data are presented as mean ± SEM of three independent experiments; *P < 0.05, **P < 0.01 compared with the Mock group; ^#P < 0.05, ^##P < 0.01 compared with the sh-Scb group