Fig. 1.

A C57BL/6 J protein restriction mouse model that combines two previously studied models with contradictory molecular consequences. a BisPCR-seq was used to simultaneously analyse methylation at CpG −133 (methylation = black circles) and genetic variation at the A/C SNP at position −104 bp in the promoter region of the 45S rDNA tandem repeats. b Small RNA-seq was used to analyse tRNA fragments derived from cleaved mature tRNAs. c Breeding and experimental scheme. Pregnant female C57BL/6 J mice were put on either a control (CT, 20% protein) or protein restricted (PR, 8% protein) diet from conception to the end of lactation. Male offspring in each litter were put on either a CT or PR diet from weaning to give four diet combinations – CTCT (black), CTPR (blue), PRCT (orange), PRPR (red). As in previously studied models, phenotype, rDNA methylation and tRNA fragment analyses were performed on sperm and other tissues taken at 84 days of age