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. 2018 Jan 22;98(3):710–716. doi: 10.4269/ajtmh.17-0404

Table 1.

Sequences of all primers used in this study

Name Sequences Function
Unspecific primers (for RT-PCR)
F3CPR 5′-GACAATTGAAGGATCTAAAATGCG-3′ Sense
R3CPR 5′-CTTTCTGCATCCAAAATATAGG-3′ Antisense
RACE primers (for 3′ cDNA isolation)
CPR3F 5′-GTGTGGCTACATCATGGTTAGC-3′ Sense
CPR3F1 5′-GACCTGGTACAGGTTTAGCTCC-3′ Sense
PCR-Primer2 5′-TAGACTTAGAAATTAATACGACTCACTATAGGCGCGCCACCG-3′ Antisense
RACE primers (for 5′ cDNA isolation)
PCR-Primer1 5′-TCATACACATACGATTTAGGTGACACTATAGAGCGGCCGCCTGCAGGAAA-3′ Sense
CPR5R 5′-TTAACTCCGAGTAGCGTGCC-3′ Antisense
CPR5RN 5′-CCGAGTAGCGTGCCAAACT-3′ Antisense
Cloning
M13F 5′-GTAAAACGACGGCCAG-3′ Sense
M13R 5′-CAGGAAACAGCTATGAC-3′ Antisense
qPCR primers (for real time PCR)
CPRRTF 5′-AACACAGATGAGGATTCGAGTAAAAA-3′ Sense
CPRRTR 5′-GTGTGCGTGGATTGGATGTTAT-3′ Antisense
QβactinaF 5′-CCCCTTTCAGTGAGGATCTTCA-3′ Sense
QβactinaR 5′-CGCCATCCTTCGATTGGA-3′ Antisense

PCR = polymerase chain reaction; qPCR = quantitative PCR; RACE = rapid amplification of cDNA end; RT-PCR = reverse transcription PCR.