Table 1.
Name | Sequences | Function |
---|---|---|
Unspecific primers (for RT-PCR) | ||
F3CPR | 5′-GACAATTGAAGGATCTAAAATGCG-3′ | Sense |
R3CPR | 5′-CTTTCTGCATCCAAAATATAGG-3′ | Antisense |
RACE primers (for 3′ cDNA isolation) | ||
CPR3F | 5′-GTGTGGCTACATCATGGTTAGC-3′ | Sense |
CPR3F1 | 5′-GACCTGGTACAGGTTTAGCTCC-3′ | Sense |
PCR-Primer2 | 5′-TAGACTTAGAAATTAATACGACTCACTATAGGCGCGCCACCG-3′ | Antisense |
RACE primers (for 5′ cDNA isolation) | ||
PCR-Primer1 | 5′-TCATACACATACGATTTAGGTGACACTATAGAGCGGCCGCCTGCAGGAAA-3′ | Sense |
CPR5R | 5′-TTAACTCCGAGTAGCGTGCC-3′ | Antisense |
CPR5RN | 5′-CCGAGTAGCGTGCCAAACT-3′ | Antisense |
Cloning | ||
M13F | 5′-GTAAAACGACGGCCAG-3′ | Sense |
M13R | 5′-CAGGAAACAGCTATGAC-3′ | Antisense |
qPCR primers (for real time PCR) | ||
CPRRTF | 5′-AACACAGATGAGGATTCGAGTAAAAA-3′ | Sense |
CPRRTR | 5′-GTGTGCGTGGATTGGATGTTAT-3′ | Antisense |
QβactinaF | 5′-CCCCTTTCAGTGAGGATCTTCA-3′ | Sense |
QβactinaR | 5′-CGCCATCCTTCGATTGGA-3′ | Antisense |
PCR = polymerase chain reaction; qPCR = quantitative PCR; RACE = rapid amplification of cDNA end; RT-PCR = reverse transcription PCR.