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Effect of nitrate and ammonium supply on isocitrate dehydrogenase transcripts in tobacco. Deveined leaves and roots were harvested after a 4-d N starvation (0 h) and after the addition of a N source (lanes 15, 30, and 96 h). mRNA was probed for IDH, ICDH1, and mtICDH. Twenty micrograms of total mRNA was loaded in each lane, which was checked by ethidium bromide staining.
