Figure 6. Details of Radical SAM enzyme AdoMet binding site.
The protein backbone is shown as grey cartoons, with AdoMet and the 4Fe-4S cluster shown in sticks (AdoMet, green carbons; iron, ruby; sulfur, gold) and core β strands labeled 1 – 6. Protein sidechains that interact with AdoMet are shown as lines with carbons colored dark grey. Hydrogen bonding contacts are shown as red (within 3.2 Å distance), green (3.2 – 3.7 Å) or yellow (more than 3.7 Å) dashed lines. Shown in this figure are the AdoMet binding sites of (a) PFL-AE (b) HemN (c) BioB (d) MoaA (e) LAM (f) the putative AdoMet binding site of phTYW1, shown with the superimposed 4Fe-4S cluster and AdoMet of the MoaA structure and (g) HydE. See Figure S8 for these images in stereoview.