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. 2018 May 2;18:495. doi: 10.1186/s12885-018-4410-x

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© The Author(s). 2018

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 3 — Specificity of the immunohistochemistry staining of colon adenocarcinoma tissue with E-Ig chimera. Images were taken with a 10× magnification, in sequences from the same tissue section of the same paraffin block of tumor tissue. Brown colour indicates positive reactivity and shows expression of E-selectin ligands (a). For control staining was performed in the absence of E-Ig (b), absence of anti-CD62E monoclonal antibody (c) and in presence of a calcium chelant - EDTA (d)