Fig. 2.

ER-c-Src-HER2 complex formation in the tamoxifen-resistant breast cancer cell line BT474. a BT474 or T47D cells (approximately 60% confluent) were treated with 17β-E2 (10 nmol/L) and/or TAM (1 μmol/L) or left untreated (vehicle control) for 4 h. Lysates were prepared and subjected to immunoblot analysis with the indicated antibodies. b BT474 and (c) T47D cells were treated with 17β-E2 (10 nmol/L) and/or tamoxifen (TAM) (1 μmol/L) for 4 h, and then lysates were immunoprecipitated using a c-Src antibody or IgG and subjected to immunoblot analysis using the indicated antibodies