Figure 1.

Fully denaturing Tris-sulfate SDS-PAGE of the fractions obtained from Suc-gradient ultracentrifugation of V. major thylakoids collected from dark-adapted (at least 12 h) leaves of GC plants. Fractions from stressed and recovered thylakoids, as well as the fractions obtained from plants grown OD, were very similar and are therefore not depicted. Whole thylakoids (T) were loaded onto the gel in addition to the four fractions (F2–F5) collected; fraction 2 contained the LHCII monomer (B), the minor Chl proteins CP29 (A), CP26 (comigrating with LHCII; B), and CP24 (C); fraction 3 contained the LHCII trimer (D); fraction 4 contained the PSII core with the D1/D2 heterodimer (E), CP47 (F), CP43 (G), and D1/D2 monomer (H); fraction 5 contained the PSI core (I) and LHCI (J). Fraction 1 contained the free pigments (Table III) and is not depicted here.