Table III.
Pigment composition of the fractions collected from Suc-gradient ultracentrifugation of solubilized thylakoids from V. major grown in GCs or OD under natural sunlight
| Pigment and Treatment | Minor CPs + LHCII Monomer (Fraction 2)
|
LHCII Trimer (Fraction 3)
|
PSII Core (Fraction 4)
|
PSI-LHCI (Fraction 5)
|
||||
|---|---|---|---|---|---|---|---|---|
| GC | OD | GC | OD | GC | OD | GC | OD | |
| mol/100 mol Chl a | ||||||||
| Chl b | 68.0 ± 1.4 | 67.8 ± 0.3 | 78.8 ± 2.0 | 74.8 ± 2.7 | 5.5 ± 1.3 | 2.8 ± 0.8 | 11.5 ± 0.5 | 11.5 ± 0.2 |
| Neoxanthin | 10.6 ± 0.7 | 10.0 ± 0.7 | 12.7 ± 0.6 | 11.3 ± 0.9 | 0.6 ± 0.5 | 0.7 ± 0.3 | 0 | 0.3 ± 0.1 |
| Lutein | 28.8 ± 0.4 | 27.6 ± 1.3 | 34.8 ± 0.5 | 31.4 ± 1.7 | 4.7 ± 0.5 | 3.1 ± 0.4 | 6.5 ± 0.3 | 5.7 ± 0.6 |
| β-Carotene | 0 | 0.4 ± 0.3 | 0 | 0 | 17.0 ± 0.1 | 12.0 ± 2.9 | 9.9 ± 0.7 | 13.6 ± 0.5 |
| V | ||||||||
| Control | 4.8 | 9.1 | 3.4 | 10.4 | 1.7 | 1.4 | 3.2 | 3.6 |
| Stress | 2.1 | 3.2 | 0.6 | 2.0 | 1.3 | 0.3 | 2.0 | 1.9 |
| Recovery | 2.5 | 5.0 | 1.0 | 3.3 | 1.0 | 0.7 | 2.3 | 2.4 |
| A | ||||||||
| Control | 0.2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
| Stress | 1.1 | 2.1 | 0.9 | 1.9 | 0.7 | 0.6 | 0.5 | 0.8 |
| Recovery | 1.4 | 3.0 | 1.0 | 1.9 | 0.9 | 0.8 | 0.6 | 0.8 |
| Z | ||||||||
| Control | 0.7 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
| Stress | 5.2 | 5.9 | 2.8 | 4.8 | 1.2 | 1.2 | 1.3 | 1.3 |
| Recovery | 4.8 | 4.3 | 2.7 | 2.5 | 1.0 | 0.9 | 1.3 | 1.7 |
| VAZ | ||||||||
| Control | 5.7 | 9.1 | 3.4 | 10.4 | 1.7 | 1.4 | 3.2 | 3.6 |
| Stress | 8.5 | 11.2 | 4.3 | 8.8 | 3.2 | 2.2 | 3.8 | 4.0 |
| Recovery | 8.7 | 12.3 | 4.7 | 7.6 | 2.8 | 2.4 | 4.2 | 4.8 |
| (Z+A)/(VAZ) | ||||||||
| Control | 0.15 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
| Stress | 0.75 | 0.68 | 0.86 | 0.74 | 0.61 | 0.82 | 0.48 | 0.47 |
| Recovery | 0.72 | 0.55 | 0.78 | 0.53 | 0.66 | 0.69 | 0.44 | 0.46 |
The pigments Chl b, neoxanthin, lutein, and β-carotene are means ± sd of the control, stress (48 h of continuous light at 15°C for leaves from GC plants and 122 h of continuous light at 10°C for leaves from plants grown OD), and recovery (2.5 h in low light at 22°C) treatments.