Table V.
Analysis of pigments in the fraction containing the PSII core (fraction 4)
| Pigment | PSII Core Suc Gradient (Fraction 4) | Calculated Value if 100% PSII Core | Calculated Values if 7% Contamination with LHCII | Actual/Calculated | Repurified Core with DEAE |
|---|---|---|---|---|---|
| mol/100 mol Chl a | |||||
| Chl b | 2.7 ± 0.8 | 0 | 2.8 | 0.92 | 1.7 ± 0.3 |
| Neoxanthin | 0.8 ± 0.3 | 0 | 0.5 | 1.53 | 0 |
| Lutein | 3.1 ± 0.4 | 2.5 | 3.55 | 0.86 | 1.0 ± 0.4 |
| β-Carotene | 12.1 ± 2.9 | 8.9 | 8.55 | 1.41 | 13.4 ± 0.9 |
| VAZ | 2.0 ± 0.5 | 0 | 0.18 | 11.17 | 0.3 ± 0.2 |
Comparison of the core fraction after Suc-gradient ultracentrifugation with values calculated based on previously published results for pure PSII core and core contaminated with 7% LHCII, in addition to pigment content after purification with DEAE-Fractogel chromatography. The data are means ± sd of the three treatments. Calculations were based on data presented by Yamamoto and Bassi (1996), except the VAZ content per LCHII was based on data from Table III.